Professor, Department of Biology
Ph.D. Brandeis University
B.S. Utah State
Research Interests: Specification and patterning of neurons and neural crest cells in embryonic zebrafish
Overview: The vertebrate nervous system is composed of a large number of neurons with diverse characteristics. My lab is interested in how neuronal diversity is generated during development: how are the correct number of cells specified for specific neuronal fates at particular times and in particular locations? Most of our attention has been focused on a small, early-developing set of individually identified spinal motoneurons and on the neural crest, a transient embryonic cell population that generates a diverse set of derivatives, including the neurons and glia of the peripheral nervous system. We use a combined cellular, molecular and genetic approach to learn the mechanisms underlying cell fate specification. For example, we study the timing of critical events during development of motoneurons and neural crest cells by labeling individual cells and following their development in living embryos and by transplanting individual cells to new locations. We are isolating genes encoding molecules that may regulate motoneuron and neural crest development and testing the roles of the proteins encoded by these genes during motoneuron and neural crest specification and differentiation. We are also isolating mutations that alter motoneuron or neural crest cell fate with the goal of identifying new genes involved in the development of these cells.
Correction: A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications.
PLoS One. 2017;12(4):e0176543
Authors: Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne P
[This corrects the article DOI: 10.1371/journal.pone.0159277.].
PMID: 28426753 [PubMed - in process]
The enteric nervous system promotes intestinal health by constraining microbiota composition.
PLoS Biol. 2017 Feb;15(2):e2000689
Authors: Rolig AS, Mittge EK, Ganz J, Troll JV, Melancon E, Wiles TJ, Alligood K, Stephens WZ, Eisen JS, Guillemin K
Sustaining a balanced intestinal microbial community is critical for maintaining intestinal health and preventing chronic inflammation. The gut is a highly dynamic environment, subject to periodic waves of peristaltic activity. We hypothesized that this dynamic environment is a prerequisite for a balanced microbial community and that the enteric nervous system (ENS), a chief regulator of physiological processes within the gut, profoundly influences gut microbiota composition. We found that zebrafish lacking an ENS due to a mutation in the Hirschsprung disease gene, sox10, develop microbiota-dependent inflammation that is transmissible between hosts. Profiling microbial communities across a spectrum of inflammatory phenotypes revealed that increased levels of inflammation were linked to an overabundance of pro-inflammatory bacterial lineages and a lack of anti-inflammatory bacterial lineages. Moreover, either administering a representative anti-inflammatory strain or restoring ENS function corrected the pathology. Thus, we demonstrate that the ENS modulates gut microbiota community membership to maintain intestinal health.
PMID: 28207737 [PubMed - in process]